|
KMID : 0894520070110030235
|
|
Development & Reproduction
2007 Volume.11 No. 3 p.235 ~ p.243
|
|
|
Neural and Cholinergic Differentiation of Mesenchymal Stem Cells Derived from the Human Umbilical Cord Blood
|
|
Kam Kyung-Yoon
Kang Ji-Hye
Do Byung-Rok
Kim Hae-kwon
Kang Sung-Goo
|
|
|
Abstract
|
|
|
|
Human umbilical cord blood(HUCB) contains a rich source of hematopoietic stem cells, mesenchymal stem cells and endothelial cell precursors. Mesenchymal stem cells(MSCs) in HUCB are multipotent stem cells, differ from hematopoietic stem cells and can be differentiated into neural cells. We studied on transdifferentiation-promoting conditions in neural cells and cholinergic neuron induction of HUCB-derived MSCs. Neural differentiation was induced by addingdimethyl sulphoxide(DMSO) and butylated hydroxyanisole(BHA) in Dulbeco’s Modified Essential Medium(DMEM) and fetal bovine serum(FBS). Differentiation of MSCs to cholinergic neurons was induced by combined treatment with basic fibroblast growth factor(bFGF), retinoic acid(RA) and sonic hedgehog(Shh). MSCs treated with DMSO and BHA rapidly assumed the morphology of multipolar neurons. Both immunocytochemistry and RT-PCR analysis indicated that the expression of a number of neural markers including β-tubulin Ⅲ, GFAP and MBP, was markedly elevated during this acute differentiation. The differentiation rate was about 32.3±2.9% for β-tubulin Ⅲ-positive cells, 11.0±0.9% for GFAP, and 9.4±1.0% for Gal-C. HUCB-MSCs treated combinatorially with bFGF, RA and Shh were differentiated into cholinergic neurons. After cholinergic neuronal differentiation, the β-tubulin Ⅲ-positive cell population of total cells was 31.3±3.2% and of differentiated neuronal population, 70.0±7.8% was ChAT-positive showing 3 folds higher in cholinergic population than neural induction. Conclusively, HUCB-derived MSCs can be differentiated into neural and cholinergic neurons and these findings suggest that HUCB are alternative cell source of treatment for neurodegenerative diseases such as Alzheimer’s disease.
|
|
|
KEYWORD
|
|
|
Human umbilical cord blood, Mesenchymal stem cells, Cholinergic neuron, Basic fibroblast growth factor, Retinoic acid, Sonic hedgehog
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|